Use of raw Euphorbia tirucalli extract for inhibition of ascitic Ehrlich tumor / Avaliação do uso do extrato bruto de Euphorbia tirucalli na inibição do tumor ascítico de ehrlich

Objective: to evaluate the effect of the Euphorbia tirucalli hydroalcoholic extract (ETHE) on the development of Ehrlich Tumor, in its ascitic form. Methods: we intraperitoneally inoculated 15 Swiss mice with 10.44 x 107 cells of Ehrlich Tumor and divided them in two groups one day after: ETHE Group (eight mice), treated with a dosage of 125 mg/kg/day of EHTE for five days; and Control Group (seven mice), treated only with 0.9% isotonic saline solution over the same period. The treatment was done by gavage. Ten days after inoculation, four mice from each group were sacrificed for quantification of tumor cell number, ascitic fluid volume and bone marrow cell number. The remaining animals were maintained to evaluate survival. Results: The ascitic fluid volume and the tumor cell number were decreased in the ETHE group when compared with the control group, but with no statistical significance. On the other hand, survival was higher in the ETHE group, as well as the number of bone marrow cells. Conclusion: Treatment with ETHE after inoculation of Ehrlich Tumor decreases its development and increases survival and the bone marrow cellularity, thus reducing the myelosuppression present in the Ehrlich Tumor bearing mice.

Objetivo: avaliar o efeito do extrato hidroalcoólico de Euphorbia tirucalli (ETHE) sobre o desenvolvimento do tumor de Ehrlich em sua forma ascítica. Métodos: quinze camundongos Swiss foram inoculados via intraperitoneal com 10,44x107 células do tumor de Ehrlich e um dia depois foram divididos em dois grupos: Grupo ETHE (oito camundongos), tratados com a dose de 125mg/kg/dia de ETHE por cinco dias e Grupo Controle (sete camundongos), tratado apenas com 0,9% de solução salina isotônica em relação ao mesmo período. O tratamento foi realizado por gavagem. Dez dias após a inoculação, quatro animais de cada grupo foram sacrificados para a quantificação do número de células de tumor, do volume de fluido ascítico e do número de células da medula óssea. Os demais animais foram mantidos, para avaliar a sobrevivência. Resultados : o volume de líquido ascítico e do número de células tumorais foram menores no grupo ETHE quando comparado ao grupo controle, porém sem significância estatística. Por outro lado, a sobrevivência dos animais foi maior no grupo de ETHE, bem como, a quantidade de células de medula óssea. Conclusão: o tratamento com ETHE, após a inoculação do tumor, diminuiu o seu desenvolvimento e aumentou sobrevida, bem como, a celularidade da medula óssea, reduzindo assim, a mielossupressão presente nos animais portadores de tumor de Ehrlich.

Sulfane sulfur deficiency in malignant cells, increasing the inhibiting action of acetone cyanohydrin in tumor growth

PURPOSE: To demonstrate the irreversible poisoning action of the acetone cyanohydrin (AC) in malignant cells. METHODS: Thirty male Swiss mice were inoculated with 1x10³ Ehrlich tumor (ET) cells. The mice were divided into three groups (n=10): CG (saline); ACG1 (1.864 mg/Kg of AC) and ACG2 (2.796 mg/Kg of AC), treated every 48 hours from day 3 until day 13. On day 15 the mice were euthanized and the number of viable cells in ascites was determined. In the meantime, ET cells were incubated with AC (0.5, 1.0, 2.0 μg/mL). Cell viability and percentage of growth inhibition (PGI) were checked after one, two, three, four, 18 and 24 hours. RESULTS: There was reduction in volume and number of viable cells in ACG1 and ACG2 compared to CG. In ACG1 one of the animals did not present ascites. In ACG2 two mice did not present ascites and in CG none of the mice present ascites. The action of AC was dose and time dependent and there was no significant difference among the three doses. CONCLUSION: The acetone cyanohydrin promoted reduction of the tumor and also prevented tumor development in 20% of the treated animals.

Influência de Agaricus blazei Murrill sobre o tumor sólido de Ehrlich e linfonodos poplíteos de camundongos / Influence of Agaricus blazei Murrill in solid Ehrlich tumor and popliteal lymph nodes in mice

Estudou-se a influência do tratamento diário com filtrado aquoso de Agaricus blazei Murrill (ABM) (25mg/ml), via oral, por 17 e 57 dias, em camundongos inoculados com tumor sólido de Ehrlich (TSE) por meio da curva de crescimento tumoral, do peso relativo do tumor, da contagem de regiões organizadoras de nucléolos (AgNORs) e dos padrões histológicos das massas tumorais e linfonodos poplíteos. Os animais que ingeriram o extrato aquoso do ABM por 57 dias apresentaram menor (P<0,05) crescimento do TSE, no segundo e terceiro dias, fase inflamatória do crescimento tumoral. Quanto ao peso relativo das massas tumorais, valores de AgNORs, padrões morfoistopatológicos do TSE e ocorrência de processos metastáticos, não houve alteração significativa (P>0,05) entre os animais tratados e não tratados.

The influence of daily administration of Agaricus blazei Murrill (ABM) aqueous solution (25mg/ml) during 17 and 57 days in mice bearing solid Ehrlich tumor was studied. Tumoral growth, tumoral and spleenic relative weights, nucleoly organization regions AgNORs values and tumor and popliteal lymph nodes histopathology were daily evaluated. The animals that received ABM during 57 days showed lower values (P<0.05) of tumoral growth on second and third days after the period corresponding to the inflammatory phase of tumoral growth. The relative weight of the tumor and AgNORs values were similar (P>0.05) between treated and non-treated animals. No difference in microscopic evaluation of the tumors in treated and non-treated animals was seen and metastasis in popliteal nodes of the tumor occurred in all the animals.

Efeito do miriadenolídeo isolado de Alomia myriadenia (Asteraceae) sobre o tumor de Erlich ascítico no camundongo / Effect of myriadenolide isolated from Alomia myriadenia (Asteraceae) on Ehrlich tumor in its ascitic form

Estudou-se atividade antineoplásica de um produto natural isolado de Alomia myriadenia (miriadenolídeo) no modelo do tumor de Ehrlich em camundongos. Dezoito fêmeas de camundongo Swiss foram inoculadas com 2x10(7) células viáveis de tumor de Ehrlich via intraperitoneal (0,3ml) e posteriormente distribuídas aleatoriamente em três grupos que receberam: grupo I (controle) - 0,3ml de solução de Hanks; grupo II - 31µg/kg de miriadenolídeo; e grupo III - 139µg/kg de miriadenolídeo. No oitavo dia de experimento, foram realizados exames hematológicos e perfil protéico sérico eletroforético. Coletou-se todo o líquido ascítico para avaliação do volume, aparência, pH, contagem de células viáveis e inviáveis, realização de esfregaços para contagem de células claras e escuras, leucócitos e avaliação das regiões organizadoras de nucléolos argentafins (AgNORs). Foram realizados exames macro e microscópicos do baço, fígado e rins e aspirado o conteúdo da medula óssea dos fêmures direito e esquerdo de cada animal para avaliação da relação mielóide:eritróide. Não houve diferença significativa no volume, pH, contagem de células viáveis e inviáveis entre os três grupos estudados, observando-se valores de 17,6 x 10(4) células tumorais viáveis no grupo III, 27,7 x 10(4) no grupo II e 21,1 x 10(4) no grupo I. As AgNORs apresentaram-se pequenas, com distribuição difusa e incontáveis no grupo I, e em menor quantidade no grupo III. Os animais do grupo III apresentaram a menor concentração protéica total sérica (4,7g/dl) (P<0,05) quando comparados com os do grupo II (5,3g/dl) e do grupo I (5,1g/dl). Os valores de albumina foram semelhantes nos três grupos (2,6g/dl), e as globulinas totais foram maiores (P<0,05) no grupo II (2,71g/dl) quando comparadas com os valores médios do grupo III (2,11g/dl) e semelhantes ao grupo I (2,43g/dl). Não houve diferença entre alfa e beta globulinas entre os três grupos estudados, porém as gamaglobulinas foram maiores...

Antitumoral activity of a natural product of Alomia myriadenia (myriadenolide) in Ehrlich tumor in mice was studied. Eighteen Swiss female mice were intra-peritoneal inoculated 2x10(7) viable cells of Ehrlich Tumor (0.3ml) and randomly distributed in three groups receiving via intra-peritoneal on the 3rd and 5th day post-inoculation the following treatments: group I (control) - 0.3ml Hanks solution; group II: 31µg/kg myriadenolide; and group III: 139µg/kg myriadenolide. On the eighth day of the experiment blood profile and protein serum electrophoresis were performed. All ascitic liquid was collected to evaluate the volume and pH; to observe the aspect; to count viable and no viable cells, dark and clear cells, leukocytes and nucleolar organizer regions (NORs). Macro and microscopic exams were performed and bone marrow was aspirated from right and left femurs of each animal to evaluate myeloid:erythroid ratio. It was not observed difference in volume, pH, counts viable and no viable cells in the groups, although group III showed smaller number of viable tumoral cells (17.6 x 10(4)) when compared to the group II (27.7 x 10(4)) and group I (21.1 x 10(4)). The investigation of NORs to evaluate the proliferative capacity of tumoral cells after myriadenolide treatment showed that cells were smaller, uncountable and with diffuse distribution in group I. They were in lower quantity in group III. These results suggest that myriadenolide in dose 139µg/kg (group III) delay the tumoral growing and, probably, cell proliferation. The animals of group III showed lower value of total protein (4.7g/dl) (P<0.05) when compared to animals from group II (5.3g/dl) and group I (5.1g/dl). The values of albumin were similar in all groups (2.6g/dl) and total globulin was higher (P<0.05) in group II (2.71g/dl) when compared to mean values of group III (2.11g/dl) and similar to group I (2.43g/dl). The decrease of total protein in group III occurred...

Marcadores de proliferação celular na avaliação do crescimento do tumor sólido e ascítico de Ehrlich / Cell proliferation markers for evaluating the growth of solid and ascitic forms of Ehrlich tumor

Twenty BALB/c mice were inoculated with cell suspension of Ehrlich tumor. Ten mice were inoculated in the cushion plant (solid form) and the other 10 in the peritoneum (ascitic form). Animals were euthanized on different times (7 and 14 days). Cytological and histological slides, immunohistochemical (PCNA) analysis and NORs silver impregnation technique were performed. The results showed more proliferation on the 7th day in the ascitic form and on 14th day in the solid form, using both analyses (PCNA and AgNORs). The alterations observed in the Ehrlich tumorÆs proliferation activity suggested that the growth curve is different between ascitic and solid forms. In the first one, the proliferation peak occurs on the seventh day and in the solid tumor the growth curve was more delayed, showing increased proliferative potential after seven days.